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Home / Biochemicals / Small Molecules

Ochratoxin A

SKU : BHB21300258

A Potent Modulator of Ca2+ ATPase Pump

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Alomone Labs

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Mfr.No.	O-400, O-400_0.5 mg, O-400_1 mg
Description	Ochratoxin A (#O-400) is a highly pure, natural, and biologically active compound.
Molecular Formula	C20H18ClNO6.
Background	Ochratoxin A is a natural metabolic alkaloid produce by Aspergillus ochraceus1. Ochratoxin A is a potent a toxin which causes nephrosis in mammals. In the circulation, Ochratoxin A has a very long half-life in all species examined2. Although the kidney is the primary target organ for Ochratoxin A activity, studies indicate that Ochratoxin A is also a liver toxin, immunosuppressant, potent teratogen, and carcinogen3. Ochratoxin A increases activity of the endoplasmic reticulum ATP-dependent Ca2+ pump4.
Shipping	Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
Concentration	100 nM - 1 uM.
Reconstitution	Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 uM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
Cas Number	303-47-9
Molecular Weight	403.8
Solubility	Methanol. Centrifuge all product preparations before use (10000 x g 5 min).
Target	SERCA pump
Source	Natural
Activity	Ochratoxin A causes an increase in ATP-driven Ca2+ pump activity in the ER1. Ochratoxin A also induces apoptosis in a JNK-dependent fashion2.
Country Of Origin	Israel/IL
UNSPSC Code	41116134
Application	FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay
Lead Time	1-2 Business Days
Notes	Chemical Name: (-)-N-((5-Chloro-8-hydroxy-3-methyl-1-oxo-7-isochromanyl)carbonyl)-3-phenylalanine.

Purity	>98%
Form	Lyophilized powder.
Storage	Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles. Storage of solutions: Up to one week at 4°C or three months at -20°C.Protect from light.

Product Manual	SDS

Reference	Van der Merwe, K.J. et al. (1965) Nature 205, 1112. Creppy, E.E. (1999) J. Toxicol. Toxin Rev. 18, 277. Beardall, J.M. and Miller, J.D. (1994) In J. D. Miller and H. L. Trenholm (ed.), Eagan Press, St. Paul, Minn. 487. Chong, X. and Rahimtula, A.D. (1992) Biochem. Pharmacol. 44, 1401. Gekle, M. et al. (2000) J. Pharmacol. Exp. Ther. 293, 837.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHB21300258-1	O-400_0.5 mg	0.5 mg	$91		Add to Cart
BHB21300258-2	O-400_1 mg	1 mg	$129		Add to Cart
BHB21300258-3	O-400_1 mg	5x1 mg	$506		Add to Cart

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