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Home / Biochemicals / Small Molecules

Oligomycin

SKU : BHB21300259

A Mitochondrial ATP Synthase Inhibitor

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Mfr.No.	O-500, O-500_1 mg, O-500_10 mg, O-500_25 mg, O-500_5 mg
Description	Oligomycin (#O-500) is a highly pure, natural, and biologically active compound.
Molecular Formula	C45H74O11 (for Oligomycin A).
Background	Oligomycin is a natural antibiotic isolated from Streptomyces diastatochromogenes which inhibits mitochondrial H+-ATP synthase. It is primarily found to act as an inhibitor of mitochondrial respiration and swelling. This antibiotic is widely used as an inhibitor of oxidative phosphorylation.1-2Oligomycin inhibits the H+- ATP-synthase by binding to the Oligomycin sensitivity-conferring protein (OSCP) at the F(o) subunits 6 and 9 which are found in the stalk of the F1F0-ATPase complex. This binding blocks the proton conductance and inhibits the synthesis of mitochondrial ATP.3-4 Because of its activity, it can also be used to reduce the number of parameters (such as ER Ca2+ release, exocytotoxicity and apopotosis) which are affected by mitochondrial depolarization.5Oligomycin, at high concentrations may also inhibit the plasma membrane Na+-K+-ATPase. Interaction of Oligomycin with the Na+ occlusion site on the extracellular side of Na/K-ATPase, delays Na+ release to the extracellular side without inducing a conformational change. Although Oligomycin stimulated Na+ binding to Na+/K+-ATPase, it inhibited Na+/Na+ exchange, and did not affect either Na+-dependent AD/-ATP exchange or K+-dependent phosphatase activity.6-16These inhibitory properties have been attributed to its ability to occlude the transition of E1~P (high energy intermediate) to E2-P (low energy intermediate) by oligomycin binding to E1~P.17
Shipping	Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
Concentration	0.1-100 uM.
Reconstitution	Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water to high-micromolar concentrations (50 uM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity. Avoid exposure to light.
Cas Number	1404-19-9
Molecular Weight	791.06
Solubility	DMSO, ethanol, acetone. Centrifuge all product preparations before use (10000 x g 5 min).
Source	Natural
Activity	Oligomycin, a natural antibiotic, inhibits mitochondrial H+-ATP synthase. It is commonly used to inhibit oxidative phosphorylation1,2.
Country Of Origin	Israel/IL
UNSPSC Code	41116134
Application	FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay
Lead Time	1-2 Business Days
Notes	Bioassay Tested: Yes Chemical Name: (1S,4E,5'R,6R,6'R,7S,8R,10S,11S,12R,14S,15R,16S,18E,20E,22S,25R,27S,28R,29S)-22-ethyl-7,11,14,15-tetrahydroxy-6'- ((2S)-2-hydroxypropyl)-5',6,8,10,12,14,16,28,29-nonamethylspiro(2,26- dioxabicyclo(23.3.1)nonacosa-4,18,20-triene-27,2'-oxane) -3,9,13-trione (for Olygomycin A).

Purity	>99%
Form	Lyophilized powder.
Storage	Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles. Storage of solutions: Up to two weeks at 4°C or six months at -20°C.

Product Manual	SDS

Reference	Chappell, J.B. and Greville, G.D. (1961) Nature 190, 502. Kagawa, Y. and Racker, E. (1966) J. Biol. Chem. 241, 2461. Kim, S.B. and Berdanier, C.D. (1999) Am. J. Physiol. Endocrinol. Metab. 277, E702. Cunningham, C.C. and George, D.T. (1975) J. Biol. Chem. 250, 2036. Arato-Oshima, T. et al. (1996) J. Biol. Chem. 271, 25604. Fortes, P.A. and Lee, J.A. (1984) J. Biol. Chem. 259, 11176. Garrahan, P.J. and Glynn, I.M. (1967) J. Physiol. 192, 217. Cornelius, F. and Skou, J.C. (1985) Biochim. Biophys. Acta. 818, 211. Martin, W.H. et al. (1994) Proc. Natl. Acad. Sci. U. S. A. 91, 9881. Hegyvary, C. (1976) Biochim. Biophys. Acta. 422, 365. Fahn, S. et al. (1966) J. Biol. Chem. 241, 1882. Fahn, S. et al. (1968) J. Biol. Chem. 243, 1993. Inturrisi, C.E. and Titus, E. (1968) Mol. Pharmacol. 4, 591. Chappell, J.B. and Greville, G.D. (1961) Nature 190, 502. Kagawa, Y. and Racker, E. (1966) J. Biol. Chem. 241, 2461.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHB21300259-1	O-500_1 mg	1 mg	$85		Add to Cart
BHB21300259-2	O-500_10 mg	10 mg	$373		Add to Cart
BHB21300259-3	O-500_25 mg	25 mg	$742		Add to Cart
BHB21300259-4	O-500_5 mg	5 mg	$235		Add to Cart

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