JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / Biochemicals / Small Molecules

RO-3

SKU : BHB21300310

A Potent and Selective Antagonist of P2X3 and P2X2/3 Receptors

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

Alomone Labs

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	R-190, R-190_10 mg, R-190_25 mg, R-190_5 mg, R-190_50 mg
Description	RO-3 (#R-190) is a highly pure, synthetic, and biologically active compound.
Molecular Formula	C16H22N4O2.
Background	RO-3 is a potent inhibitor of human homomultimeric P2X3 receptor and heteromultimeric P2X2/3 receptor and demonstrates pIC50 value of 7.0 and pIC50=5.9 respectively1.The compound shows to have to high metabolic stability in rat and human hepatocytes and liver microsomes. It's highly permeable, orally bioavailable, and has a good in vivo plasma half-life in rats. RO-3 is also widely distributed to tissues after administration, with low plasma protein binding and good CNS penetration1.The P2X receptors are a family of ion channels that are gated by ATP. P2X receptors are widely localized in cell types of almost every origin, including neuronal, muscular, epithelial, and immune and have been shown to play a pivotal role in models of various pain conditions2.
Shipping	Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
Concentration	50 nM - 50 uM.
Reconstitution	Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 uM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity
Cas Number	1026582-88-6
Molecular Weight	302.37
Solubility	Up to 100 mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Target	P2X3 receptors
Source	Synthetic
Activity	RO-3 is a potent and selective antagonist of the P2X3 and P2X2/3 receptors. It inhibits human homomeric P2X3 and heteromeric P2X2/3 receptors with pIC50 values of 7.0 and 5.9, respectively, whilst showing selectivity over other homomeric P2X family receptors with pIC50 of < 51.
Country Of Origin	Israel/IL
UNSPSC Code	41116134
Application	FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay
Lead Time	1-2 Business Days
Notes	Bioassay Tested: Yes Chemical Name: 5-((4,5-dimethoxy-2-propan-2-ylphenyl)methyl)pyrimidine-2,4-diamine.

Purity	>97% (HPLC)
Form	Lyophilized powder.
Storage	Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles. Storage of solutions: -20°C.

Product Manual	SDS

Reference	Ford, A. et al. (2006) Br. J. Pharmacol. 147, S132. Pijacka, W. et al. (2016) Nat. Med. 22, 1151.

Have you used this product?

Rate it now..!!

Login to Review

Combination SKU	Supplier No.	Size	Your price	Quantity
BHB21300310-1	R-190_10 mg	10 mg	$202		Add to Cart
BHB21300310-2	R-190_25 mg	25 mg	$451		Add to Cart
BHB21300310-3	R-190_5 mg	5 mg	$168		Add to Cart
BHB21300310-4	R-190_50 mg	50 mg	$808		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2025 Biohippo. All rights reserved.