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Home / Biochemicals / Small Molecules

TRAM 34

SKU : BHB21300335

A Selective and Potent Blocker of KCa3.1 Channels

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Mfr.No.	T-105, T-105_10 mg, T-105_25 mg, T-105_5 mg, T-105_50 mg
Description	TRAM 34 (#T-105) is a highly pure, synthetic, and biologically active compound.
Molecular Formula	C22H17ClN2.
Synonyms	Synthetic
Background	KCa3.1 (SK4, intermediate conductance Ca2+ activated K+) is part of the Ca2+ activated K+ channel family that share the characteristic of being activated by intracellular Ca2+. The channel has an intermediate conductance, is voltage insensitive and is activated by Ca2+ in the submicromolar range. The channel has a similar topology to that of KV channels, that is six transmembrane domains and intracellular N- and C-termini. KCa3.1 is widely expressed in epithelial, endothelial cells and cells of hematopoietic origin. In erythrocytes (red blood cells) it has been identified as the molecular correlate of the so-called Gardos channel1.TRAM 34 is a potent and selective KCa3.1 channel inhibitor; 50 nM blocks KCa3.1 channel expressed in COS-7 cells by over 50%2. As such, it is a powerful pharmacological tool that enables the determination of the roles played by KCa3.1 channels in different physiological systems, ranging from activation of T lymphocytes to migration of endothelial cells3-4.By using TRAM 34 it was demonstrated that proliferation of vascular endothelial cells and angiogenesis in vivo are mediated via KCa3.1 channels4.in vivo delivery of TRAM 34 via balloon catheter prevented porcine coronary smooth muscle phenotypic modulation and limited subsequent vasculoproliferative diseases5.10 mM TRAM 34, completely stopped proliferation of the human pancreatic cancer cell lines, (BxPC-3 MiaPaCa-2) while proliferation of PANC-1 cells was hardly affected by TRAM 34, which was well correlated to the elevated levels of KCa3.1 expression in the former as well as in samples from pancreatic cancer patients6.
Shipping	Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
Concentration	10 nM - 1 uM.
Reconstitution	Centrifuge the vial before adding solvent (10,000 x g for 5 minutes). The lyophilizate may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. Soluble in any conventional buffer containing 0.01 KH2PO4, 0.1% gelatin and/or 0.1% bovine serum albumin (BSA). The use of gelatin or 0.01% BSA coated/treated glassware is recommended. Centrifuge all product preparations before use (10000 x g 5 min).
Cas Number	289905-88-0
Molecular Weight	344.8
Solubility	DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Target	KCa3.1 K+ channels
Activity	TRAM 34 is a potent and selective KCa3.1 (SK4, intermediate conductance Ca2+-activated K+) channel inhibitor1. As such, it is a powerful pharmacological tool that enables the determination of the roles played by KCa3.1 channels in different physiological systems ranging from activation of T lymphocytes to migration of endothelial cells2-3.
Country Of Origin	Israel/IL
UNSPSC Code	41116134
Application	FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay
Lead Time	1-2 Business Days
Notes	Bioassay Tested: Yes Cited Application: Electrophysiology Chemical Name: 1-((2-Chlorophenyl)diphenylmethyl)-1H-pyrazole.

Purity	>99%
Form	Lyophilized Powder
Storage	Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles. Storage of solutions: Up to four weeks at 4°C or three months at -20°C.

Product Manual	SDS

Reference	Gutman, G. A. et al. Calcium-Activated Potassium Channels. Last modified on 2011-01-31. Accessed on 2011-06-22. IUPHAR database (IUPHAR-DB). Wulff, H. et al. (2000) Proc. Natl. Acad. Sci. U.S.A. 97, 8151. Beeton, C. et al. (2001) Proc. Natl. Acad. Sci. U.S.A. 98, 13942. Grgic, I. et al. (2005) Arterioscler. Thromb. Vasc. Biol. 25, 704. Tharp, D.L. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28, 1084. Jager, H. et al. (2004) Mol. Pharmacol. 65, 630. Wulff, H. et al. (2000) Proc. Natl. Acad. Sci. U.S.A. 97, 8151. Beeton, C. et al. (2001) Proc. Natl. Acad. Sci. U.S.A. 98, 13942. Grgic, I. et al. (2005) Arterioscler. Thromb. Vasc. Biol. 25, 704.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHB21300335-1	T-105_10 mg	10 mg	$110		Add to Cart
BHB21300335-2	T-105_25 mg	25 mg	$250		Add to Cart
BHB21300335-3	T-105_5 mg	5 mg	$81		Add to Cart
BHB21300335-4	T-105_50 mg	50 mg	$306		Add to Cart

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