Matrices listed below were spiked with certain level of IL5 and the recovery rates were calculated by comparing the measured value to the expected amount of IL5 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-100
90
EDTA plasma(n=5)
87-104
97
heparin plasma(n=5)
88-105
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-103%
87-96%
88-102%
EDTA plasma(n=5)
83-94%
84-100%
84-95%
heparin plasma(n=5)
86-98%
83-96%
86-98%
Sensitivity
< 4.688pg/ml
Notes
For Research Use Only
UniProt ID
P52173
Antigen
IL5
Applications
ELISA
Applications Notes
IL5 ELISA Kit allows for the in vitro quantitative determination of IL5 concentrations in serum, plasma, tissue homogenates and other biological fluids.