Mouse Anti-Human c-fos Monoclonal IgG2b

Mfr.No.	SMC-545, SMC-545D, SMC-545D-A390, SMC-545D-A488, SMC-545D-A594, SMC-545D-ALP, SMC-545D-APC, SMC-545D-BI, SMC-545D-FITC, SMC-545D-HRP, SMC-545D-PCP, SMC-545D-RPE, SMC-545S
Immunogen Species	Human
Alternative Names	c-fos Antibody, Activator protein 1 antibody, AP 1 antibody, C FOS antibody, Cellular oncogene c fos antibody, Cellular oncogene fos antibody, FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS) antibody, FBJ murine osteosarcoma viral oncogene homolog antibody, FBJ murine osteosarcoma viral v fos oncogene homolog antibody, FBJ Osteosarcoma Virus antibody, FOS antibody, FOS protein antibody, FOS_HUMAN antibody, G0 G1 switch regulatory protein 7 antibody, G0/G1 switch regulatory protein 7 antibody, G0S7 antibody, Oncogene FOS antibody, p55 antibody, proto oncogene c Fos antibody, Proto oncogene protein c fos antibody, Proto-oncogene c-Fos antibody, v fos FBJ murine osteosarcoma viral oncogene homolog antibody
Antigen	c-fos
Categories	Antibodies
Cellular Localization	Endoplasmic reticulum, Cytosol, Nucleus
Certificate Of Analysis	A 1:1000 dilution of SMC-545 was sufficient for detection of c-fos in 10 ug of serum starved HeLa cells treated with PMA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Clonality	Monoclonal
Conjugate	Unconjugated, ATTO 390, ATTO 488, ATTO 594, Alkaline Phosphatase, APC, Biotin, FITC, HRP, PerCP, RPE
Description	Mouse Anti-Human c-fos Monoclonal IgG2b
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Mouse
Gross Weight (kg)	1.4
Immunogen	Synthetic peptide from the N-terminal of Human c-Fos (aa. 280-380), Homo Sapiens (Human)
Immunogen Region	Synthetic peptide from the N-terminal of Human c-Fos (aa. 280-380)
Isotype	IgG2b
Launch Date	2017/10/10
Net Weight (g)	0.1
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Human
Tissue Specificity	Expressed at very low levels in quiescent cells. When cells are stimulated to reenter growth, they undergo 2 waves of expression, the first one peaks 7.5 minutes following FBS induction. At this stage, the protein is localized endoplasmic reticulum. The second wave of expression occurs at about 20 minutes after induction and peaks at 1 hour. At this stage, the protein becomes nuclear.

Swissprot	P01100
Accession Number	NP_005243.1
Gene Id	2353
Target	c-fos
Clone No	5B11
Research Area	Cell Markers, Neuron Markers, Cell Signaling, Epigenetics and Nuclear Signaling, Transcription, Domain Families, Cancer, Tumor Biomarkers, Oncoproteins, Chromatin Modifying Enzymes, Neuroscience, Cytoplasmic Markers

Buffer	PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Concentration	1 mg/ml
Dilutions	WB (1:1000); optimal dilutions for assays should be determined by the user.
Purification	Protein G Purified
Purity	Expressed at very low levels in quiescent cells. When cells are stimulated to reenter growth, they undergo 2 waves of expression, the first one peaks 7.5 minutes following FBS induction. At this stage, the protein is localized endoplasmic reticulum. The second wave of expression occurs at about 20 minutes after induction and peaks at 1 hour. At this stage, the protein becomes nuclear.
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects 62 kDa band. Observed molecular weight of band is higher than predicted 41 kDa band due to post translational modifications.
Storage	-20ºC
Storage Buffer	PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	WB
Description	WB
Dilution	WB (1:1000); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11906015-1	SMC-545D	Unconjugated	100 ug	$330	Add to Cart
BHA11906015-2	SMC-545D-A390	ATTO 390	100 ug	$384	Add to Cart
BHA11906015-3	SMC-545D-A488	ATTO 488	100 ug	$383	Add to Cart
BHA11906015-4	SMC-545D-A594	ATTO 594	100 ug	$383	Add to Cart
BHA11906015-5	SMC-545D-ALP	Alkaline Phosphatase	100 ug	$376	Add to Cart
BHA11906015-6	SMC-545D-APC	APC	100 ug	$382	Add to Cart
BHA11906015-7	SMC-545D-BI	Biotin	100 ug	$378	Add to Cart
BHA11906015-8	SMC-545D-FITC	FITC	100 ug	$374	Add to Cart
BHA11906015-9	SMC-545D-HRP	HRP	100 ug	$370	Add to Cart
BHA11906015-10	SMC-545D-PCP	PerCP	100 ug	$382	Add to Cart
BHA11906015-11	SMC-545D-RPE	RPE	100 ug	$380	Add to Cart
BHA11906015-12	SMC-545S	Unconjugated	12 ug	$44	Add to Cart

c-Fos Antibody, Clone 5B11 - SMC-545

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