5-hydroxytryptamine (serotonin) receptor 7B cell line

Mfr.No.	AG-10200-240
Description	cAMP assay for Gs-coupled human 5-hydroxytryptamine (serotonin) receptor 7B (HTR7B). HEK293-CNG cells (AG-10200-200) without transfected 5-hydroxytryptamine (serotonin) receptor 7B are used as a negative control.
Gene Name	Human 5-hydroxytryptamine (serotonin) receptor 7B (HTR7B)
Cell Origin	Human
Culture Medium	Growth medium for maintaining the cells: 90% DMEM, 10% FBS, 250 µg/ml G418 and 1 µg/ml puromycin Growth medium for ACTOne Assay: 90% DMEM, 10% FBS (CHARCOAL STRIPPED), 250 µg/ml G418 and 1 µg/ml puromycin Freezing medium: 10% DMSO, 90% complete medium

Figure 1. Response of ACTOne HTR7B cell line & parental cell line to 5-HT. ACTOne 5-hydroxytryptamine (serotonin) receptor 7B cells and parental cells (AG-10200-200) were plated overnight in 20 ml culture medium on a BD Biocoat 384 well plate. The next day, cells were dye-loaded with 20 ml/well of 1X Dye-loading solution (ACTOne Membrane Potential Assay Kit). After 2 hours of incubation at room temperature, two readings were obtained prior to and 30 min after the addition of 5-HT. Ratios of the two readings (F/F0) are plotted in the figure. A. Dose response curve of 5-HT in ACTOne 5-hydroxytryptamine (serotonin) receptor 7B cell line. EC50 = 28 nM in the presence of PDE inhibitor Ro20-1724. B. Parental cells do not respond to 5-HT.

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5-hydroxytryptamine (serotonin) receptor 7B cell line

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