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Human Primary Diabetic Pulmonary Vein Smooth Muscle Cells (HDPVSM)

SKU : BHC16400142

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Cell Biologics, Inc.

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Mfr.No.	HD2-6084
Description	Human Primary Diabetic Pulmonary Vein Smooth Muscle Cells from Cell Biologics are isolated from the pulmonary vein of human donor that has been diagnosed with diabetes type II disease. Human Primary Diabetic Pulmonary Vein Smooth Muscle Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Cell Biologics Culture Complete Growth Medium generally for 3-7 days. Prior to shipping, cells at passage 1 are detached from the culture flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10⁶ cells per ml and is delivered frozen. The cells are characterized by immunofluorescent method with antibodies to ?-smooth muscle actin. Human Primary Diabetic Pulmonary Vein Smooth Muscle Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded on a multiwell culture plate ready for experiments (or cells may be expanded for 2-4 passages at a split ratio of 1:2) under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.
Cell Origin	Human
Cell Type	Smooth Muscle Cells
Application	Human Primary Diabetic Pulmonary Vein Smooth Muscle Cells can be used in assays of standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Disclaimer	Appropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from Cell Biologics with caution and treat all cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http://www.cdc.gov/biosafety/publicati ons/bmbl5/index.htm .
Frozen	0.5×10⁶cells
General Information	Human Primary Diabetic Pulmonary Vein Smooth Muscle Cells can be used in assays of standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Tissue	Pulmonary Vein

Product Use	Human Primary Diabetic Pulmonary Vein Smooth Muscle Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures. Transfer or resale of any Cell Biologics cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the companys written consent. Cell Biologics Terms and Conditions must be accepted before submitting an order.
Storage	Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live-cell shipment is also available on request. Primary cells should never be kept at -20 °C

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SKU	Supplier No.	Size	Your price	Quantity
BHC16400142	HD2-6084	Frozen Vial	$720		Add to Cart

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