Mouse Mesenchymal Stem Cells - Bone Marrow (MMSC-bm)

Product Description

Mesenchymal stem cells (MSC) derived from bone marrow are a well-characterized population of adult stem cells. MSC have the capability for renewal and differentiation into various lineages of mature cells that produce fat, cartilage, bone,tendons, and muscle. These properties, in combination with their developmental plasticity, have generated tremendous interest in regenerative medicine to replace damaged tissues. These findings have spurred the development of MSC-basedtherapies for treating wide range of non-skeletal diseases ^1,2 .

iXCells Biotechnologies offers mBM-MSC isolated from mouse bone marrow. Each vialcontains >0.5 million cells. These cells are expanded in Mesenchymal Stem Cell Medium (Cat# MD-0037) and then cryopreserved at passage 2. iXCells mBM-MSC characterized by immunofluorescence and flow cytometry arestrongly positive for MSC marker CD29 (Figure 1). These cells can further be differentiated into adipocytes using Adipocyte Differentiation Medium (Cat# MD-0005) and into osteoblastsusing Osteogenic Differentiation Medium (Cat# MD-0006) (Figure 2 and Figure 3). mBM-MSC are negative for mycoplasma, bacteria, yeast, and fungi and can be expanded for no more than 3 passages in iXCells Mesenchymal Stem Cell Medium. 

 Figure 1. (A) Phase contrast image of MMSC-bm (B) Immunofluorescence staining with antibody against    CD29. (C) Flow cytometric analysis shows >90% CD29 positive cells. 

Figure 2. MMSC-bm adipocyte differentiation (Day 14 post adipogenic induction).     

Figure 3. MMSC-bm osteogenic differentiation (Day 27 post osteogenic induction). Alizarin Red S staining of osteoblasts. The extracellular calcium deposit was stained in bright orange-red color.

Product Details 

Reference

(1) Huang S, Xu L, Sun Y, Wu T, Wang K, Li G. An improved protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow. JOrthop Translat. 2014, 27;3(1):26-33.

(2) García-Gómez I, Elvira G, Zapata AG, Lamana ML, Ramírez M, Castro JG, Arranz MG, Vicente A, Bueren J, García-Olmo D. Mesenchymal stem cells:biological properties and clinical applications. Expert Opin Biol Ther. 2010,10(10):1453-68.

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