Bright -One Step Luciferase Assay Kit

Firefly luciferase is a protein with a molecular weight of approximately 61 kDa. In the presence of ATP, magnesium ions, and oxygen, it can catalyze the oxidation of luciferin to oxyluciferin, during which a bioluminescence with a wavelength of around 560 nm is emitted.

Detection principle as shown in the figure:

Figure 1: Schematic diagram of the firefly luciferase detection principle

YEASEN Biotech's Bright-One Step Luciferase Assay Kit features easy operation, high sensitivity, and strong signal intensity. It is suitable for experiments that require high sensitivity. There is no need to wash or collect cells before the experiment, and it can be used directly. The lysis and detection of cells are completed in one step, eliminating the need to mix reagents, saving experimental time.

Features

Long signal half-life: The signal half-life is 30 min, meeting the needs of high-throughput experiments.

Strong fluorescence signal: The signal value can reach 8 orders of magnitude, with a large detection window.

Convenient operation: One-step procedure, avoiding complicated experimental processes.

Applications

Signaling Pathway Research

Candidate Drug Screening

T Cell Killing Assay

Specifications

Components

Shipping and Storage

Bright -One Step Luciferase Assay Kit products should be stored at -80? for 1 years.

Figures

Figure1. YEASEN Bright -One Step has better stability and higher signal values compared to its competitors.

Documents:

Safety Data Sheet

11412_MSDS_HB241231_EN.PDF

 Manuals

11412_Manual_HB241230_EN.pdf