Pyrophosphatase, Inorganic GMP-grade (0.1 U/μL)

Mfr.No.

10672ES

Description

This product is an inorganic pyrophosphatase derived from recombinant expression in E. coli. It is an enzyme that can catalyze the conversion of one molecule of pyrophosphate into two molecules of phosphate ions. This is a high-energy reaction, so this reaction can be coupled to some thermodynamically unfavorable transformations to drive these transformations to completion. Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate. In molecular biology, it can be used to increase RNA yield in reverse transcription reactions.

This product is produced by GMP process requirements, and the product is provided in liquid form

Unit Definition
One unit (U) is the amount of enzyme needed to catalyze the hydrolysis of PPI per minute to produce 1 μmol Pi.（The standard reaction is: 500 μL system contains 100 mM Tris-HCl pH7.2, 2 mM MgCl2, 2 mM Ppi; reaction at 25℃ for 10 min）

Kit Components

Catalog No.	Name	Catalog No./Specification
		10672ES03 (1 U)	10672ES10 (10 U)	10672ES60 (100 U)
10672	Pyrophosphatase, Inorganic GMP-grade (0.1 U/μL)	10 μL	100 μL	1 mL

Notes

1. This product is active in a variety of reaction buffers. Usually, this product can be directly connected in experiments such as IVT.
2. The optimal reaction temperature of this product is 25℃, it is active at 16-37℃, and 65℃ can inactivate the enzyme for 10 minutes.
3. For your safety and health, please wear personal protective equipment (PPE), such as laboratory coats and disposable gloves, when operating with this

Source

Recombinant E.coli with Pyrophosphatase gene from yeast

Shipping	The Pyrophosphatase, Inorganic GMP-grade products are shipped with dry ice.
Storage	can be stored at -15℃ ~ -25℃ for one year.
Storage Buffer	50 mM Tris-HCl pH 7.9, 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 0.1% （v/v）TritonX-100, 50%（v/v）glycerin
Storage Temperature	-15℃ ~ -25℃

Product Manual	10672-Pyrophosphatase,Inorganic GMP-grade-HB221008.pdf

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHS20800378-1	10672ES10	10 U	$70		Add to Cart
BHS20800378-2	10672ES60	100 U	$475		Add to Cart

Pyrophosphatase, Inorganic GMP-grade (0.1 U/μL)

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