Bovine Interferon gamma ,IFN-gamma ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E09812b

Alternative Names

interferon, gamma, IFG, IFI,IFN-gamma,interferon gamma

Reactivity

Bovine

Detection Range

31.25 pg/mL-2000 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human sCD146 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	93-99
EDTA plasma (n=4)	98	95-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human sCD146 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	85
	Range %	81-90
1:10	Average %	96
	Range %	93-99
1:20	Average %	87
	Range %	83-90
1:40	Average %	104
	Range %	101-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Bos taurus

Sensitivity

7.8 pg/mL

Limit Of Detection

450 nm

UniProt ID	P07353
Antigen	interferon, gamma

Applications

ELISA

Research Area

IFNG

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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