Canine Parathyroid Hormone

Mfr.No.

E0320Ca

Sensitivity

4.99ng/L

Detection Range

10-2000ng/L

Reactivity

Canine

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Parathyrin;Parathyroid hormone;PTH

Molecular Weight

12957

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E0320Ca within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	243	14.51	6
Intra-Assay	2	18	788.7	28.34	3.6
Intra-Assay	3	18	465.9	24.6	5.3

Background

PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates (1-14C)-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells (By similarity).

Protocol

These standard curves of E0320Ca are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.35	2.228	2.174
	2.106
600ng/L	1.302	1.315	1.262
	1.328
300ng/L	0.758	0.812	0.758
	0.867
150ng/L	0.47	0.512	0.458
	0.554
75ng/L	0.243	0.236	0.182
	0.229
0ng/L	0.064	0.054	0
	0.044

Gene ID	403986
UniProt ID	P52212

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100828	E0320Ca	96T	$475		Add to Cart
BHE12100828	E0320Ca	48T	$350		Add to Cart

Canine Parathyroid Hormone - PTH ELISA Kit

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