Chicken Corticosterone, CORT ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E11991C

Sensitivity

0.5 ng/mL

Detection Range

0.5 ng/mL-20 ng/mL

Reactivity

Chicken

Assay Duration

1-5h

Assay Type

Competitive

Sample Volume

50-100ul

Alternative Names

Corticosterone, CORT

Recovery

The recovery of bovine APOA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	82-87
EDTA plasma (n=4)	89	85-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine APOA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1000	Average %	90
	Range %	87-94
1:2000	Average %	96
	Range %	91-100
1:4000	Average %	93
	Range %	87-97
1:8000	Average %	100
	Range %	96-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Gallus gallus

Sample Type

Serum, Plasma

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	CORT

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Creatine Monohydrate Enhances Energy Status and Reduces Glycolysis via Inhibition of AMPK Pathway in Pectoralis Major Muscle of Transport-Stressed Broilers Zhang L.et al,J Agric Food Chem.,Aug 8 Forsythia suspensa extract attenuates breast muscle oxidative injury induced by transport stress in broilers Pan L.et al,Poult Sci,Mar 8 Forsythia suspensa extract protects broilers against breast muscle oxidative injury induced by corticosterone mimicked pre-slaughter acute stress Pan L.et al,Poult Sci,Mar 5 Dietary L-theanine alleviated lipopolysaccharide-induced immunological stress in yellow-feathered broilers RuiLi.et al,Animal Nutrition,May 23

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BHE10500421	CSB-E11991C	10x96T	$8132	Add to Cart