Human Plasminogen Activator, Urokinase (uPA) (CLIA) ELISA Kit

Mfr.No.

SCA140Hu

Sensitivity

1.24pg/mL

Detection Range

2.74-2,000pg/mL

Reactivity

Human

Sample Type

Cell lysates, Cell culture supernates, Serum, Tissue homogenates, Plasma, Other biological fluids

Assay Duration

2h, 40min

Assay Type

Sandwich

Description

This assay has high sensitivity and excellent specificity for detection of Plasminogen Activator, Urokinase (uPA).
No significant cross-reactivity or interference between Plasminogen Activator, Urokinase (uPA) and analogues was observed.

Alternative Names

PLAU; ATF; URK; UK; UP-A; Abbokinase; Urokinase-Type Plasminogen Activator

Detection Method

Chemiluminescence

Kit Components

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1×120µL	Assay Diluent A	1×12mL
Detection Reagent B	1×120µL	Assay Diluent B	1×12mL
Substrate A	1×10mL	Substrate B	1×2mL
Wash Buffer (30 × concentrate)	1×20mL	Instruction manual	1

Recovery

Matrices listed below were spiked with certain level of recombinant Plasminogen Activator, Urokinase (uPA) and the recovery rates were calculated by comparing the measured value to the expected amount of Plasminogen Activator, Urokinase (uPA) in samples.

Matrix	Recovery range (%)	Average(%)
sodium citrate plasma(n=5)	97-105	101

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Plasminogen Activator, Urokinase (uPA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8	1:16
sodium citrate plasma(n=5)	81-97%	96-103%	84-94%	81-105%

Features

Detects protein (regular version)

Species

Human

Precautions

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Plasminogen Activator, Urokinase (uPA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Plasminogen Activator, Urokinase (uPA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: C<10%
Inter-Assay: C<12%

Notes

The microplate provided in this kit has been pre-coated with an antibody specific to Plasminogen Activator, Urokinase (uPA). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Plasminogen Activator, Urokinase (uPA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Plasminogen Activator, Urokinase (uPA) level in the sample or standard.;

Usage Notes

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Protocol

Datasheet

Gene ID	5328
UniProt ID	P00749
Antigen	uPA

Applications	ELISA
Research Area	Tumor immunity

Storage	4°C for 1 month/ -20°C for long-term(within shelf life)
Shelf Life	one year

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SKU	Supplier No.	Size	Your price
BHE10101070	SCA140Hu	96T*1	$840	Add to Cart
BHE10101070	SCA140Hu	96T*5	$3780	Add to Cart
BHE10101070	SCA140Hu	96T*10	$7140	Add to Cart

CLIA Kit for Plasminogen Activator, Urokinase (uPA) (Human)

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