EasyStep Human FE(Ferritin) ELISA Kit

Mfr.No.

ELK017ES

Sensitivity

3.2 pg/mL

Detection Range

0.313 ng/mL - 20 ng/mL

Reactivity

Human

Assay Duration

1.5h

Assay Type

Sandwich

Alternative Names

Ferritin

Recovery

Matrices listed below were spiked with certain level of recombinant FE and the recovery rates were calculated by comparing the measured value to the expected amount of FE in samples.

Matrix	Recovery range	Average
serum(n=5)	95-107%	101%
EDTA plasma(n=5)	85-95%	90%
Heparin plasma(n=5)	79-93%	86%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	95-103%	89-101%	87-98%	85-92%
EDTA plasma(n=5)	86-96%	85-97%	89-101%	85-97%
Heparin plasma(n=5)	89-97%	85-94%	79-96%	85-99%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
20.00	2.075	1.987
10.00	1.515	1.427
5.00	1.174	1.086
2.50	0.828	0.740
1.25	0.453	0.365
0.63	0.426	0.338
0.32	0.252	0.164
0.00	0.088	0.000

Sample Type

Saliva, Cell lysates, Serum, Cell culture supernatants, Tissue homogenates, Urine, Cerebrospinal fluid, Plasma, Other biological fluids

Limit Of Detection

20 ng/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FE, and the Human FE standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human FE. After TMB substrate solution is added, only those wells that contain Human FE and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FE in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

Applications	ELISA
Research Area	Metabolic pathway;Tumor immunity;Hematology;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15208340	ELK017ES	48T	$412		Add to Cart
BHE15208340	ELK017ES	96T	$588		Add to Cart

EasyStep Human FE(Ferritin) ELISA Kit

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