General species Anti-5-Hydroxytryptamine Antibody (Anti-5-HT) (Competitive EIA) ELISA Kit

Mfr.No.

AEA808Ge

Description

This assay has high sensitivity and excellent specificity for detection of Anti-5-Hydroxytryptamine Antibody (Anti-5-HT).
No significant cross-reactivity or interference between Anti-5-Hydroxytryptamine Antibody (Anti-5-HT) and analogues was observed.

Alternative Names

5HT; Serotonin; N-Methyl-Gamma

Reactivity

General

Detection Method

Colormetric

Detection Range

3.12-200ng/mL

Sample Type

Other biological fluids, Serum, Plasma

Assay Type

Competitive Inhibition

Assay Duration

2h, 30min

Kit Components

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1×120µL	Assay Diluent A	1×12mL
TMB Substrate	1×9mL	Stop Solution	1×6mL
Wash Buffer (30 × concentrate)	1×20mL	Instruction manual	1

Recovery

Matrices listed below were spiked with certain level of Anti-5-Hydroxytryptamine Antibody (Anti-5-HT) and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-5-Hydroxytryptamine Antibody (Anti-5-HT) in samples.

Matrix	Recovery range (%)	Average(%)
serum(n=5)	78-92	84
EDTA plasma(n=5)	95-104	101
heparin plasma(n=5)	79-104	87

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-5-Hydroxytryptamine Antibody (Anti-5-HT) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8	1:16
serum(n=5)	79-99%	92-102%	91-101%	79-96%
EDTA plasma(n=5)	78-99%	90-101%	90-104%	94-104%
heparin plasma(n=5)	78-94%	78-95%	79-92%	96-103%

Features

Detects protein (antigen)

Species

General species

Sensitivity

1.13ng/mL

Precautions

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Notes

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-5-Hydroxytryptamine Antibody (Anti-5-HT) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-5-Hydroxytryptamine Antibody (Anti-5-HT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Antigen	5-HT
Usage Notes	1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 5 times; 5. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 6. Add 50µL Stop Solution. Read at 450nm immediately.

Applications	ELISA
Research Area	Signal transduction
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-5-Hydroxytryptamine Antibody (Anti-5-HT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-5-Hydroxytryptamine Antibody (Anti-5-HT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: C<10% Inter-Assay: C<12%

Storage	4°C for 1 month/ -20°C for long-term(within shelf life)
Shelf Life	one year

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SKU	Supplier No.	Size	Your price
BHE10109608	AEA808Ge	96T*1	$850	Add to Cart
BHE10109608	AEA808Ge	96T*5	$3825	Add to Cart
BHE10109608	AEA808Ge	96T*10	$7225	Add to Cart

ELISA Kit for Anti-5-Hydroxytryptamine Antibody (Anti-5-HT) (General species)

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