Fish Interleukin-8,IL-8 ELISA Kit

Mfr.No.

CSB-E12877Fh

Sensitivity

31.25 pg/mL

Detection Range

31.25 pg/mL-500 pg/mL

Assay Duration

1-5h

Assay Type

Competitive

Sample Volume

50-100ul

Alternative Names

Interleukin-8,IL-8

Recovery

The recovery of human ADAMDEC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	99-108
EDTA plasma (n=4)	94	89-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ADAMDEC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-96
1:2	Average %	96
	Range %	90-101
1:4	Average %	94
	Range %	89-99
1:8	Average %	97
	Range %	92-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Fish

Sample Type

Serum, Plasma

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	Q6F4N2

Applications	ELISA
Research Area	IL8, CXCL8

Storage	Store at 2-8°C. Please refer to protocol.

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