Matrices listed below were spiked with certain level of AKT3 and the recovery rates were calculated by comparing the measured value to the expected amount of AKT3 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-100
92
EDTA plasma(n=5)
87-98
92
heparin plasma(n=5)
85-101
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AKT3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
92-103%
87-102%
94-104%
EDTA plasma(n=5)
86-100%
87-98%
84-94%
heparin plasma(n=5)
82-100%
80-87%
83-93%
Sensitivity
< 0.188ng/ml
Notes
For Research Use Only
UniProt ID
Q9Y243
Antigen
AKT3
Applications
ELISA
Applications Notes
AKT3 ELISA Kit allows for the in vitro quantitative determination of AKT3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human AKT3(Protein kinase Akt-3) ELISA Kit