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Home / ELISA Kit

Human Alcohol dehydrogenase 1B (ADH1B) ELISA Kit

SKU : BHE13703152

ADH2 encoded by this gene is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This encoded protein, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism.
Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. Previously ADH1B was called ADH2. There are more genes in the family of alcohol and aldehyde dehydrogenase genes. These genes are now referred to as ADH1A, ADH1C, and ADH4, ADH5, ADH6 and ADH7.

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Mfr.No.	KTE60936
Description	This Human Alcohol dehydrogenase 1B (ADH1B) ELISA Kit employs a two-site sandwich ELISA to quantitate ADH1B.
Alternative Names	ADH1B; ADH2; DKFZp686C06125; ADH; beta subunit; alcohol dehydrogenase 2 (class I); beta polypeptide; aldehyde reductase; class I alcohol dehydrogenase; beta subunit
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Alcohol dehydrogenase 1B microplate Human Alcohol dehydrogenase 1B standard Human Alcohol dehydrogenase 1B detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Alcohol dehydrogenase 1B (ADH1B) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADH1B. No significant cross-reactivity or interference between Human ADH1B and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	ADH2 encoded by this gene is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This encoded protein, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. Previously ADH1B was called ADH2. There are more genes in the family of alcohol and aldehyde dehydrogenase genes. These genes are now referred to as ADH1A, ADH1C, and ADH4, ADH5, ADH6 and ADH7.
Notes	This Human Alcohol dehydrogenase 1B (ADH1B) ELISA Kit employs a two-site sandwich ELISA to quantitate ADH1B in samples. An antibody specific for ADH1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADH1B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADH1B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADH1B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	125
Antigen	ADH1B
Tag	ADH1B
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Alcohol dehydrogenase 1B (ADH1B) ELISA Kit employs a two-site sandwich ELISA to quantitate ADH1B in samples. An antibody specific for ADH1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADH1B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADH1B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADH1B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703152	KTE60936	48 T	$339		Add to Cart
BHE13703152	KTE60936	96 T	$589		Add to Cart
BHE13703152	KTE60936	96 Tx5	$2629		Add to Cart
BHE13703152	KTE60936	96 Tx50	$24569		Add to Cart

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