Human Angiotensin I Converting Enzyme,ACEI ELISA Kit

Human Angiotensin I Converting Enzyme,ACEI ELISA Kit

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Mfr.No.

CSB-E11269h

Sensitivity

4.7 pg/mL

Detection Range

18.75 pg/mL-1200 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

angiotensin I converting enzyme (peptidyl-dipeptidase A) 1, ACE1, CD143, DCP, DCP1, MGC26566, MVCD3, CD143 antigen, angiotensin I converting enzyme 1, angiotensin converting enzyme, somatic isoform, carboxycathepsin, dipeptidyl carboxypeptidase 1, kininase II, pep, Angiotensin I Converting Enzyme,ACEI

Recovery

The recovery of human VEGF121 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-104
EDTA plasma (n=4)	91	87-95

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human VEGF121 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	98
	Range %	92-106
1:2	Average %	94
	Range %	90-98
1:4	Average %	89
	Range %	86-96
1:8	Average %	92
	Range %	86-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.415	2.314	2.365	2.222
5	1.532	1.633	1.583	1.440
2.5	0.972	0.951	0.962	0.819
1.25	0.558	0.547	0.553	0.410
0.625	0.404	0.411	0.408	0.265
0.312	0.285	0.274	0.280	0.137
0.156	0.226	0.220	0.223	0.080
0	0.143	0.142	0.143	?

Species

Homo sapiens

Sample Type

Serum, Cell culture supeatants, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P12821
Tissue Specificity	Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis.

Applications	ELISA
Research Area	ACE

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501190	CSB-E11269h	96T	$595	Add to Cart
BHE10501190	CSB-E11269h	5x96T	$2856	Add to Cart
BHE10501190	CSB-E11269h	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.