Human Anti-C1q(Anti-Complement 1q Antibody) ELISA Kit

Mfr.No.

ELK7843

Sensitivity

0.58 ng/mL

Detection Range

1.57-100 ng/mL

Reactivity

Human

Assay Duration

2.5h

Assay Type

Competitive Inhibition

Alternative Names

C1-q Ab

Recovery

Matrices listed below were spiked with certain level of recombinant Anti-C1q and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-C1q in samples.

Matrix	Recovery range	Average
serum(n=5)	86-99%	92%
EDTA plasma(n=5)	82-97%	89%
Heparin plasma(n=5)	80-95%	87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-C1q and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	87-96%	85-97%	85-94%	87-96%
EDTA plasma(n=5)	93-104%	83-96%	95-103%	88-95%
Heparin plasma(n=5)	89-102%	92-101%	88-102%	87-102%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
100.00	0.183
50.00	0.310
25.00	0.451
12.50	0.846
6.25	1.389
3.13	1.439
1.57	1.761
0.00	2.316

Sample Type

Serum, Plasma, Other biological fluids

Limit Of Detection

100 ng/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Anti-C1q protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-C1q. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-C1q in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

Applications	ELISA
Research Area	Infection immunity;Immune molecule;Autoimmunity;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15200465	ELK7843	48T	$320		Add to Cart
BHE15200465	ELK7843	96T	$458		Add to Cart

Human Anti-C1q(Anti-Complement 1q Antibody) ELISA Kit

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