Human anti-respiratory syncytial virus(RSV) antibody (IgM) ELISA Kit

Mfr.No.

CSB-E13790h

Alternative Names

anti-respiratory syncytial virus(RSV) antibody (IgM)

Reactivity

Human

Detection Range

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Sample Type

Serum

Assay Type

Indirect

Assay Duration

1-5h

Recovery

The recovery of human YBX1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	101-109
EDTA plasma (n=4)	97	93-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human YBX1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	81-93
1:2	Average %	102
	Range %	95-109
1:4	Average %	95
	Range %	91-99
1:8	Average %	86
	Range %	83-91

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.658	2.698	2.678	2.575
1000	2.381	2.451	2.416	2.313
500	1.974	1.962	1.968	1.865
250	1.229	1.169	1.199	1.096
125	0.698	0.687	0.693	0.590
62.5	0.400	0.412	0.406	0.303
31.25	0.209	0.211	0.210	0.107
0	0.101	0.105	0.103	?

Species

Homo sapiens

Sensitivity

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Limit Of Detection

450 nm

Antigen	anti-respiratory syncytial virus(RSV) antibody (IgM)

Applications

ELISA

Research Area

IgM

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Real time-PCR, ELISA, as an identification methods for Detection respiratory syncytial virus in children Suad H.et al,TIKRIT JOURNAL OF PURE SCIENCE,/ 11.DETECTION OF RESPIRATORY SYNCYTIAL VIRUS IN INFANTS AND YOUNG CHILDREN WITH CHEST INFECTION: A COMPARISON OF REVERSE TRANSCRIPTION-PCR TECHNIQUE TO CHROMATOGRAPHIC IMMUNOASSAY AND ENZYME LINKED IMMUNOSORBENT ASSAY AMA Al-Shuwaikh, et al,IRAQI JOURNAL OF MEDICAL SCIENCES,/

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SKU	Supplier No.	Size	Your price
BHE10501339	CSB-E13790h	96T	$595	Add to Cart
BHE10501339	CSB-E13790h	5x96T	$2856	Add to Cart
BHE10501339	CSB-E13790h	10x96T	$5652.50	Add to Cart

Human anti-respiratory syncytial virus(RSV) antibody (IgM) ELISA Kit

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