Human Anti-THSD7A(Anti-Thrombospondin Type I Domain Containing Protein 7A Antibody) ELISA Kit

Mfr.No.

ELK8249

Sensitivity

1.22 ng/mL

Detection Range

3.13-200 ng/mL

Reactivity

Human

Assay Duration

2.5h

Assay Type

Competitive Inhibition

Alternative Names

THS7A; Thsd7a

Recovery

Matrices listed below were spiked with certain level of recombinant Anti-THSD7A and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-THSD7A in samples.

Matrix	Recovery range	Average
serum(n=5)	87-99%	93%
EDTA plasma(n=5)	87-95%	91%
Heparin plasma(n=5)	78-93%	85%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-THSD7A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	88-97%	91-103%	86-92%	89-97%
EDTA plasma(n=5)	85-94%	87-96%	89-101%	82-90%
Heparin plasma(n=5)	87-101%	87-96%	82-93%	89-97%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
200.00	0.197
100.00	0.397
50.00	0.546
25.00	0.833
12.50	1.169
6.25	1.589
3.13	1.715
0.00	2.213

Sample Type

Serum, Plasma, Other biological fluids

Limit Of Detection

200 ng/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Anti-THSD7A protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-THSD7A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-THSD7A in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

Applications	ELISA
Research Area	Signal transduction;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15200333	ELK8249	48T	$320		Add to Cart
BHE15200333	ELK8249	96T	$458		Add to Cart

Human Anti-THSD7A(Anti-Thrombospondin Type I Domain Containing Protein 7A Antibody) ELISA Kit

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