Human ASAH1(N-Acylsphingosine Amidohydrolase 1) ELISA Kit

Mfr.No.

ELK2966

Sensitivity

0.63 ng/mL

Detection Range

1.57-100 ng/mL

Reactivity

Human

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

AC; ASAH; PHP; PHP32; Acid Ceramidase; Acylsphingosine deacylase; Putative 32 kDa heart protein

Recovery

Matrices listed below were spiked with certain level of recombinant ASAH1 and the recovery rates were calculated by comparing the measured value to the expected amount of ASAH1 in samples.

Matrix	Recovery range	Average
serum(n=5)	80-97%	88%
EDTA plasma(n=5)	80-95%	87%
Heparin plasma(n=5)	83-95%	89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ASAH1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	88-104%	84-98%	95-105%	88-102%
EDTA plasma(n=5)	86-93%	91-103%	87-98%	92-99%
Heparin plasma(n=5)	86-93%	91-105%	87-98%	89-94%

Typical Data

<table> <tbody><tr> <th>Concentration (ng/mL)</th> <th>OD</th> <th>Corrected OD</th> </tr> <tr> <td>100.00</td> <td>2.135</td> <td>2.052</td> </tr> <tr> <td>50.00</td> <td>1.562</td> <td>1.479</td> </tr> <tr> <td>25.00</td> <td>1.143</td> <td>1.060</td> </tr> <tr> <td>12.50</td> <td>0.615</td> <td>0.532</td> </tr> <tr> <td>6.25</td> <td>0.549</td> <td>0.466</td> </tr> <tr> <td>3.13</td> <td>0.352</td> <td>0.269</td> </tr> <tr> <td>1.57</td> <td>0.224</td> <td>0.141</td> </tr> <tr> <td>0.00</td> <td>0.083</td> <td>0.000</td> </tr> </tbody></table>

Sample Type

Cell lysates, Tissue homogenates, Other biological fluids

Limit Of Detection

100 ng/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ASAH1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ASAH1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ASAH1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ASAH1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

<a href="https://s3.us-west-1.amazonaws.com/s3.biohippo.com/elisakits/elk/ELK2966-1.pdf" target="_blank">Datasheet</a>

UniProt ID	Q13510

Applications	ELISA
Research Area	Enzyme & Kinase;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15203286	ELK2966	48T	$320		Add to Cart
BHE15203286	ELK2966	96T	$458		Add to Cart

Human ASAH1(N-Acylsphingosine Amidohydrolase 1) ELISA Kit

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