Human beta-site APP-Cleaving Enzyme 1,BACE1 ELISA kit

Mfr.No.

CSB-E09824h

Sensitivity

0.78 pg/mL

Detection Range

3.12 pg/mL-200 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

beta-site APP-cleaving enzyme 1, ASP2, BACE, FLJ90568, HSPC104, KIAA1149, APP beta-secretase, aspartyl protease 2, beta-secretase, beta-site amyloid beta A4 precursor protein-cleaving enzyme, beta-site amyloid betaA4 precursor protein-cleaving enzyme, beta-site amyloid precursor protein cleavin, beta-site amyloid precursor protein cleavin, beta-site APP-Cleaving Enzyme 1,BACE1, beta-site APP-Cleaving Enzyme 1,BACE1

Recovery

The recovery of human FGF-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	84-92
EDTA plasma (n=4)	104	100-109

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human FGF-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-90
1:2	Average %	96
	Range %	92-100
1:4	Average %	90
	Range %	85-94
1:8	Average %	92
	Range %	88-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	2.152	2.263	2.208	2.062
400	1.607	1.712	1.660	1.514
200	1.179	1.096	1.138	0.992
100	0.781	0.745	0.763	0.617
50	0.435	0.451	0.443	0.297
25	0.314	0.307	0.311	0.165
12.5	0.228	0.234	0.231	0.085
0	0.143	0.149	0.146	?

Species

Homo sapiens

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P56817
Tissue Specificity	Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata.

Applications	ELISA
Research Area	BACE1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504932	CSB-E09824h	96T	$790	Add to Cart
BHE10504932	CSB-E09824h	5x96T	$3792	Add to Cart
BHE10504932	CSB-E09824h	10x96T	$7505	Add to Cart

Human beta-site APP-Cleaving Enzyme 1,BACE1 ELISA kit

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