Human Betaine--Homocysteine S-methyltransferase 1

Mfr.No.

E4176hu

Sensitivity

10.31ng/L

Detection Range

20-4500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Betaine–homocysteine S-methyltransferase 1 (also known as BHMT) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Betaine--homocysteine S-methyltransferase 1;BHMT

Molecular Weight

44998

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E4176hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	370.4	19.64	5.3
Intra-Assay	2	18	614.1	32.13	5.2
Intra-Assay	3	18	1522.7	53.05	3.5

Background

Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.

Protocol

These standard curves of E4176hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.385	2.421	2.371
	2.457
1200ng/L	1.487	1.463	1.413
	1.438
600ng/L	0.715	0.723	0.672
	0.73
300ng/L	0.392	0.388	0.338
	0.384
150ng/L	0.198	0.226	0.176
	0.254
0ng/L	0.038	0.05	0
	0.062

Gene ID	635
UniProt ID	Q93088

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105576	E4176hu	48T	$320		Add to Cart
BHE12105576	E4176hu	96T	$458		Add to Cart

Human Betaine--Homocysteine S-methyltransferase 1 - BHMT ELISA Kit

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