Human CD6(T-cell differentiation antigen CD6) ELISA Kit

Mfr.No.

ELK9910

Sensitivity

11.5 pg/mL

Detection Range

31.25-2000 pg/mL

Assay Duration

3.5h

Assay Type

Sandwich

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CD6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	92-106%	93-105%	85-99%	90-99%
EDTA Plasma(n=5)	91-99%	83-89%	88-104%	97-103%
Heparin Plasma(n=5)	85-97%	86-102%	87-99%	99-105%

Standard Curve

Concentration (pg/mL)	OD	Corrected OD
2000.00	2.126	2.037
1000.00	1.562	1.473
500.00	1.253	1.164
250.00	0.872	0.783
125.00	0.567	0.478
62.50	0.335	0.246
31.25	0.226	0.137
0.00	0.089	0.000

Sample Type

Serum, Plasma, tissue homogenates and other biological fluids

Limit Of Detection

2000 pg/mL

Precision

Matrices listed below were spiked with certain level of recombinant CD6 and the recovery rates were calculated by comparing the measured value to the expected amount of CD6 in samples.

Matrix	Recovery range	Average
serum(n=5)	83-95%	89%
EDTA Plasma(n=5)	90-105%	97%
Heparin Plasma(n=5)	80-97%	88%

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CD6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CD6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CD6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CD6 in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Manual
MSDS

UniProt ID	P30203

Research Area	Signal transduction;Infection immunity;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15210388	ELK9910	48T	$320		Add to Cart
BHE15210388	ELK9910	96T	$458		Add to Cart

Human CD6(T-cell differentiation antigen CD6) ELISA Kit

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