Human cholecystokinin,CCK ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08116h

Sensitivity

20 pg/mL

Detection Range

56 pg/mL-800 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Recovery

The recovery of rat folic acid spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	86-102
EDTA plasma (n=4)	94	88-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat folic acid in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	86-104
1:2	Average %	92
	Range %	83-99
1:4	Average %	96
	Range %	87-98
1:8	Average %	94
	Range %	80-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P06307

Applications	ELISA
Research Area	CCK

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10501772	CSB-E08116h	96T	$790	Add to Cart
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BHE10501772	CSB-E08116h	10x96T	$7505	Add to Cart