Human Chymotrypsin-like Elastase Family Member 1

Mfr.No.

E5049Hu

Sensitivity

0.17ng/mL

Detection Range

0.3-90ng/mL

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Chymotrypsin-like elastase family member 1;ELA1;Elastase-1;Pancreatic elastase 1

Molecular Weight

27798

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E5049Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	27.5	1.23	4.47
Intra-Assay	2	18	12.36	0.67	5.43
Intra-Assay	3	18	19.46	1.02	5.23

Background

Chymotrypsin-like elastase family member 1 (CELA1) also known as elastase-1 (ELA1) is an enzyme that in humans is encoded by the CELA1 gene. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B.

Protocol

These standard curves of E5049Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48ng/mL	1.999	2.041	1.988
	2.083
24ng/mL	1.359	1.394	1.341
	1.429
12ng/mL	0.731	0.66	0.606
	0.588
6ng/mL	0.362	0.411	0.358
	0.461
3ng/mL	0.118	0.128	0.075
	0.137
0ng/mL	0.059	0.053	0
	0.047

Gene ID	1990
UniProt ID	Q9UNI1

Research Area	Cell Biology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106412	E5049Hu	48T	$320		Add to Cart
BHE12106412	E5049Hu	96T	$458		Add to Cart

Human Chymotrypsin-like Elastase Family Member 1 - ELA1 ELISA Kit

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