Human CoII2-1(Degradation Product of Type-II Collagen) ELISA Kit

Mfr.No.

ELK0096

Sensitivity

18.2 pg/mL

Detection Range

46.88-3000 pg/mL

Assay Duration

3.5h

Assay Type

Sandwich

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CoII2-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	86-95%	93-103%	85-94%	87-101%
EDTA Plasma(n=5)	79-93%	86-92%	84-98%	89-102%
Heparin Plasma(n=5)	90-99%	87-103%	78-91%	83-97%

Standard Curve

Concentration (pg/mL)	OD	Corrected OD
3000.00	2.219	2.138
1500.00	1.586	1.505
750.00	1.034	0.953
375.00	0.828	0.747
187.50	0.477	0.396
93.75	0.363	0.282
46.88	0.247	0.166
0.00	0.081	0.000

Sample Type

Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates, Other biological fluids

Limit Of Detection

3000 pg/mL

Precision

Matrices listed below were spiked with certain level of recombinant CoII2-1 and the recovery rates were calculated by comparing the measured value to the expected amount of CoII2-1 in samples.

Matrix	Recovery range	Average
serum(n=5)	80-95%	87%
EDTA Plasma(n=5)	78-96%	87%
Heparin Plasma(n=5)	78-92%	85%

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CoII2-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CoII2-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CoII2-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CoII2-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Manual
MSDS

Research Area	Metabolic pathway;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15208981	ELK0096	48T	$265		Add to Cart
BHE15208981	ELK0096	96T	$376		Add to Cart

Human CoII2-1(Degradation Product of Type-II Collagen) ELISA Kit

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