Human Complement Factor D

Mfr.No.

E0319Hu

Sensitivity

0.21ng/ml

Detection Range

0.5-100ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Complement Factor D/Adipsin (also known as CFD) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Adipsin;C3 convertase activator;CFD;Complement factor D;Properdin factor D

Molecular Weight

27033

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E0319Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	42.1	1.28	3
Intra-Assay	2	18	10.2	0.54	5.3
Intra-Assay	3	18	21.8	1.47	6.7

Background

Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.

Protocol

These standard curves of E0319Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
64ng/ml	2.277	2.326	2.257
	2.374
32ng/ml	1.392	1.319	1.25
	1.246
16ng/ml	0.848	0.781	0.712
	0.714
8ng/ml	0.434	0.456	0.387
	0.478
4ng/ml	0.31	0.285	0.216
	0.26
0ng/ml	0.076	0.069	0
	0.063

Gene ID	1675
UniProt ID	P00746

Research Area	Immunology

Storage	2-8°C

Reference	Adipocytokines and the risk of coronary heart disease in healthy middle aged men: the PRIME Study Journal: Int J Obes (Lond). 2010 Jan;34(1):118-26

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102024	E0319Hu	96T	$458		Add to Cart
BHE12102024	E0319Hu	48T	$320		Add to Cart

Human Complement Factor D - CFD ELISA Kit

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