Matrices listed below were spiked with certain level of CRLF2 and the recovery rates were calculated by comparing the measured value to the expected amount of CRLF2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-103
91
EDTA plasma(n=5)
87-104
98
heparin plasma(n=5)
87-104
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CRLF2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
91-103%
86-90%
91-102%
EDTA plasma(n=5)
82-98%
90-101%
83-93%
heparin plasma(n=5)
81-100%
81-98%
82-99%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
Q9HC73
Applications
ELISA
Applications Notes
CRLF2 ELISA Kit allows for the in vitro quantitative determination of CRLF2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human CRLF2 (Cardiotrophin-like cytokine factor 2) ELISA Kit