Human Defensin-6(DEFA6) ELISA kit

Mfr.No.

CSB-EL006658HU

Alternative Names

defensin, alpha 6, Paneth cell-specific, DEF6, HD-6, defensin 6, defensin, alpha 6

Reactivity

Human

Detection Range

0.156 ng/mL-10 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse NRG4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-93
EDTA plasma (n=4)	94	90-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse NRG4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-103
1:2	Average %	91
	Range %	84-96
1:4	Average %	90
	Range %	87-95
1:8	Average %	100
	Range %	95-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

0.039 ng/mL

Limit Of Detection

450 nm

UniProt ID	Q01524
Antigen	defensin, alpha 6, Paneth cell-specific

Applications

ELISA

Research Area

DEFA6

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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