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Home / ELISA Kit

Human Defensin-6 - DEFA6 ELISA Kit

SKU : BHE12105470

Has very low antimicrobial activity against Gram-negative and Gram-positive bacteria. May protect cells against infection with HIV-1.

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+1 866.986.9598

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Mfr.No.

E4051hu

Sensitivity

0.024ng/ml

Detection Range

0.05-20ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

DEFA 6;DEFA6;Defensin, alpha 6;Defensin-6

Molecular Weight

10975

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E4051hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1.9	0.14	7.1
Intra-Assay	2	18	5.3	0.23	4.3
Intra-Assay	3	18	1.2	0.08	6.4

Background

Has very low antimicrobial activity against Gram-negative and Gram-positive bacteria. May protect cells against infection with HIV-1.

Protocol

These standard curves of E4051hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.312	2.337	2.292
	2.362
6ng/ml	1.696	1.66	1.616
	1.624
3ng/ml	0.957	0.992	0.947
	1.026
1.5ng/ml	0.753	0.75	0.705
	0.747
0.75ng/ml	0.407	0.421	0.376
	0.434
0ng/ml	0.037	0.045	0
	0.052

Gene ID	1671
UniProt ID	Q01524

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105470	E4051hu	48T	$320		Add to Cart
BHE12105470	E4051hu	96T	$458		Add to Cart

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