Human EMR1(EGF Like Module Containing Mucin Like Hormone Receptor 1) ELISA Kit

Mfr.No.

ELK4795

Sensitivity

25.4 pg/mL

Detection Range

62.5-4000 pg/mL

Reactivity

Human

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

TM7LN3; EGF-like module receptor 1; EMR1 hormone receptor

Recovery

Matrices listed below were spiked with certain level of recombinant EMR1 and the recovery rates were calculated by comparing the measured value to the expected amount of EMR1 in samples.

Matrix	Recovery range	Average
serum(n=5)	96-109%	102%
EDTA plasma(n=5)	81-95%	88%
Heparin plasma(n=5)	85-97%	91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of EMR1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	88-97%	95-106%	83-98%	93-105%
EDTA plasma(n=5)	88-101%	90-98%	92-106%	85-97%
Heparin plasma(n=5)	95-104%	82-96%	92-103%	90-99%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
4000.00	2.268	2.175
2000.00	1.642	1.549
1000.00	1.205	1.112
500.00	0.826	0.733
250.00	0.509	0.416
125.00	0.302	0.209
62.50	0.225	0.132
0.00	0.093	0.000

Sample Type

Cell lysates, Tissue homogenates, Other biological fluids

Limit Of Detection

4000 pg/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EMR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EMR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EMR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EMR1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

UniProt ID	Q14246

Applications	ELISA
Research Area	CD & Adhesion molecule;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15201813	ELK4795	48T	$320		Add to Cart
BHE15201813	ELK4795	96T	$458		Add to Cart

Human EMR1(EGF Like Module Containing Mucin Like Hormone Receptor 1) ELISA Kit

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