Human FABP3(Fatty Acid Binding Protein 3, Muscle And Heart) ELISA Kit

Mfr.No.

ELK1826

Sensitivity

0.251 ng/mL

Detection Range

0.63-40 ng/mL

Reactivity

Human

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

MDGI; FABP11; H-FABP; O-FABP; Hfabp; Mammary Derived Growth Inhibitor; Fatty Acid Binding Protein 11; Muscle fatty acid-binding protein; Heart-type fatty acid-binding protein

Recovery

Matrices listed below were spiked with certain level of recombinant FABP3 and the recovery rates were calculated by comparing the measured value to the expected amount of FABP3 in samples.

Matrix	Recovery range	Average
serum(n=5)	80-94%	87%
EDTA plasma(n=5)	89-101%	95%
Heparin plasma(n=5)	82-95%	88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FABP3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	85-92%	96-104%	90-98%	80-92%
EDTA plasma(n=5)	88-97%	86-92%	95-103%	89-97%
Heparin plasma(n=5)	79-94%	87-96%	82-95%	89-102%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
40.00	1.952	1.859
20.00	1.643	1.550
10.00	1.121	1.028
5.00	0.826	0.733
2.50	0.457	0.364
1.25	0.326	0.233
0.63	0.192	0.099
0.00	0.093	0.000

Sample Type

Cell lysates, Serum, Cell culture supernatants, Tissue homogenates, Plasma, Other biological fluids

Limit Of Detection

40 ng/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FABP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FABP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FABP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FABP3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

UniProt ID	P05413

Applications	ELISA
Research Area	Metabolic pathway;Cardiovascular biology;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15203864	ELK1826	48T	$320		Add to Cart
BHE15203864	ELK1826	96T	$458		Add to Cart

Human FABP3(Fatty Acid Binding Protein 3, Muscle And Heart) ELISA Kit

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