Human FcgR3A(Fc Fragment Of IgG Low Affinity IIIa Receptor) ELISA Kit

Mfr.No.

ELK3839

Sensitivity

50 ng/mL

Detection Range

31.25-2000 ng/mL

Reactivity

Human

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

CD16a; CD16-A; FCGR3-A; FCG3; FcgRIII; IGFR3; NA; Neutrophil Antigen; Low affinity immunoglobulin gamma Fc region receptor III-A

Recovery

Matrices listed below were spiked with certain level of recombinant FcgR3A and the recovery rates were calculated by comparing the measured value to the expected amount of FcgR3A in samples.

Matrix	Recovery range	Average
serum(n=5)	93-107%	100%
EDTA plasma(n=5)	87-99%	93%
Heparin plasma(n=5)	80-93%	86%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FcgR3A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	87-98%	83-94%	85-98%	85-97%
EDTA plasma(n=5)	86-102%	87-103%	85-101%	81-99%
Heparin plasma(n=5)	93-102%	93-106%	87-101%	91-104%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
2000.00	2.270	2.201
1000.00	1.687	1.618
500.00	1.128	1.059
250.00	0.861	0.792
125.00	0.453	0.384
62.50	0.319	0.250
31.25	0.175	0.106
0.00	0.069	0.000

Sample Type

Cell lysates, Serum, Tissue homogenates, Plasma, Other biological fluids

Limit Of Detection

2000 ng/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FcgR3A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FcgR3A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FcgR3A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FcgR3A in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

UniProt ID	P08637

Applications	ELISA
Research Area	CD & Adhesion molecule;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15202588	ELK3839	48T	$320		Add to Cart
BHE15202588	ELK3839	96T	$458		Add to Cart

Human FcgR3A(Fc Fragment Of IgG Low Affinity IIIa Receptor) ELISA Kit

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