Human Fibroblast Growth Factor-binding Protein 3

Mfr.No.

E6500Hu

Sensitivity

0.015ng/ml

Detection Range

0.05-15ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

FGF-binding protein 3;FGFBP 3;FGFBP3;FGFBP-3;FGF-BP3;Fibroblast growth factor-binding protein 3

Molecular Weight

27590

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E6500Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1	0.06	6.2
Intra-Assay	2	18	6	0.17	2.9
Intra-Assay	3	18	2.7	0.13	4.7

Background

Heparin-binding protein which binds to FGF2, prevents binding of FGF2 to heparin and probably inhibits immobilization of FGF2 on extracellular matrix glycosaminoglycans, allowing its release and subsequent activation of FGFR signaling which leads to increased vascular permeability.

Protocol

These standard curves of E6500Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
8ng/ml	2.435	2.309	2.232
	2.184
4ng/ml	1.312	1.295	1.218
	1.277
2ng/ml	0.8	0.82	0.743
	0.841
1ng/ml	0.406	0.42	0.343
	0.433
0.5ng/ml	0.27	0.28	0.203
	0.29
0ng/ml	0.077	0.077	0
	0.077

Gene ID	143282
UniProt ID	Q8TAT2

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115654	E6500Hu	48T	$320		Add to Cart
BHE12115654	E6500Hu	96T	$458		Add to Cart

Human Fibroblast Growth Factor-binding Protein 3 - FGFBP3 ELISA Kit

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