Human Gamma-Glutamyl Hydrolase

Mfr.No.

E4544Hu

Sensitivity

0.094ng/ml

Detection Range

0.2-60ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Conjugase;Gamma-glutamyl hydrolase;Gamma-Glu-X carboxypeptidase;GGH;GH

Molecular Weight

35964

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E4544Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	5	0.24	4.8
Intra-Assay	2	18	15.4	0.44	2.8
Intra-Assay	3	18	9.1	0.45	5

Background

Hydrolyzes the polyglutamate sidechains of pteroylpolyglutamates. Progressively removes gamma-glutamyl residues from pteroylpoly-gamma-glutamate to yield pteroyl-alpha-glutamate (folic acid) and free glutamate. May play an important role in the bioavailability of dietary pteroylpolyglutamates and in the metabolism of pteroylpolyglutamates and antifolates.

Protocol

These standard curves of E4544Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
32ng/ml	2.546	2.509	2.463
	2.472
16ng/ml	1.486	1.438	1.392
	1.391
8ng/ml	0.807	0.878	0.832
	0.949
4ng/ml	0.387	0.428	0.382
	0.469
2ng/ml	0.189	0.19	0.143
	0.191
0ng/ml	0.049	0.047	0
	0.044

Gene ID	8836
UniProt ID	Q92820

Research Area	Metabolism

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105859	E4544Hu	48T	$320		Add to Cart
BHE12105859	E4544Hu	96T	$458		Add to Cart

Human Gamma-Glutamyl Hydrolase - GGH ELISA Kit

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