Human HCN4(Hyperpolarization Activated Cyclic Nucleotide Gated Potassium Channel 4) ELISA Kit

Mfr.No.

ELK5355

Sensitivity

0.122 ng/mL

Detection Range

0.32-20 ng/mL

Reactivity

Human

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

HCN4

Recovery

Matrices listed below were spiked with certain level of recombinant HCN4 and the recovery rates were calculated by comparing the measured value to the expected amount of HCN4 in samples.

Matrix	Recovery range	Average
serum(n=5)	95-107%	101%
EDTA plasma(n=5)	78-95%	86%
Heparin plasma(n=5)	82-95%	88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HCN4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	98-107%	93-102%	80-93%	91-100%
EDTA plasma(n=5)	85-96%	87-101%	89-102%	85-98%
Heparin plasma(n=5)	86-101%	86-101%	87-96%	92-105%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
20.00	1.908	1.821
10.00	1.711	1.624
5.00	1.175	1.088
2.50	0.796	0.709
1.25	0.574	0.487
0.63	0.315	0.228
0.32	0.191	0.104
0.00	0.087	0.000

Sample Type

Tissue homogenates, Other biological fluids

Limit Of Detection

20 ng/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HCN4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HCN4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HCN4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HCN4 in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

UniProt ID	Q9Y3Q4

Applications	ELISA
Research Area	Signal transduction;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15201450	ELK5355	48T	$320		Add to Cart
BHE15201450	ELK5355	96T	$458		Add to Cart

Human HCN4(Hyperpolarization Activated Cyclic Nucleotide Gated Potassium Channel 4) ELISA Kit

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