Human High Affinity Immunoglobulin Epsilon Receptor Subunit Alpha

Mfr.No.

E5401Hu

Sensitivity

1.62ng/L

Detection Range

3-900ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Fc-epsilon RI-alpha;FCER1A;FcERI;High affinity immunoglobulin epsilon receptor subunit alpha;IgE Fc receptor subunit alpha

Molecular Weight

29596

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E5401Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	260.7	9.78	3.8
Intra-Assay	2	18	73.7	3.39	4.6
Intra-Assay	3	18	137.8	6.22	4.5

Background

Binds to the Fc region of immunoglobulins epsilon. High affinity receptor. Responsible for initiating the allergic response. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines.

Protocol

These standard curves of E5401Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
480ng/L	2.025	2.069	2.042
	2.113
240ng/L	1.428	1.442	1.415
	1.456
120ng/L	0.734	0.712	0.686
	0.691
60ng/L	0.443	0.382	0.356
	0.322
30ng/L	0.196	0.157	0.13
	0.118
0ng/L	0.024	0.026	0
	0.029

Gene ID	2205
UniProt ID	P12319

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106763	E5401Hu	48T	$320		Add to Cart
BHE12106763	E5401Hu	96T	$458		Add to Cart

Human High Affinity Immunoglobulin Epsilon Receptor Subunit Alpha - FCER1A ELISA Kit

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