Matrices listed below were spiked with certain level of HTR2A and the recovery rates were calculated by comparing the measured value to the expected amount of HTR2A in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-105
95
EDTA plasma(n=5)
89-105
96
heparin plasma(n=5)
86-99
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HTR2A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-98%
87-104%
85-104%
EDTA plasma(n=5)
86-95%
84-93%
83-101%
heparin plasma(n=5)
85-97%
81-100%
80-100%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
P28223
Applications
ELISA
Applications Notes
HTR2A ELISA Kit allows for the in vitro quantitative determination of HTR2A concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human HTR2A (5-hydroxytryptamine receptor 2A) ELISA Kit