Human Interleukin 12,IL-12/P40 ELISA KIT

Human Interleukin 12,IL-12/P40 ELISA KIT

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Mfr.No.

CSB-E04598h

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/ml-10 ng/ml

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40), CLMF, CLMF2, IL-12B, NKSF, NKSF2, IL12, subunit p40, interleukin 12, p40, interleukin 12B, interleukin-12 beta chain, natural killer cell stimulatory factor, 40 kD subunit

Recovery

The recovery of mouse TNF-? antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-94
EDTA plasma (n=4)	92	87-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TNF-? antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-92
1:2	Average %	97
	Range %	90-105
1:4	Average %	87-96
	Range %	82-96
1:8	Average %	93
	Range %	88-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.713	2.752	2.733	2.580
5	2.212	2.349	2.281	2.128
2.5	1.514	1.623	1.569	1.416
1.25	0.849	0.864	0.857	0.704
0.625	0.502	0.525	0.514	0.361
0.312	0.313	0.316	0.315	0.162
0.156	0.257	0.270	0.264	0.111
0	0.148	0.157	0.153	?

Species

Homo sapiens

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P29460

Applications	ELISA
Research Area	IL12B

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
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BHE10502941	CSB-E04598h	10x96T	$4702.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.