Human Interleukin 21,IL-21 ELISA Kit

Mfr.No.

CSB-E11707h

Sensitivity

0.78 pg/mL

Detection Range

3.12 pg/mL-200 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

interleukin 21, IL-21, Za11, interleukin-21 isoform

Recovery

The recovery of mouse TGAB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-103
EDTA plasma (n=4)	95	90-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TGAB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-101
1:2	Average %	89
	Range %	85-98
1:4	Average %	92
	Range %	84-100
1:8	Average %	95
	Range %	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	Q9HBE4
Tissue Specificity	Expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes.

Applications	ELISA
Research Area	IL21

Storage	Store at 2-8°C. Please refer to protocol.

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