Human KIF5B(Kinesin Family Member 5B) ELISA Kit

Mfr.No.

ELK0377

Sensitivity

30.9 pg/mL

Detection Range

78.13-5000 pg/mL

Assay Duration

3.5h

Assay Type

Sandwich

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of KIF5B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	80-97%	81-92%	87-103%	82-93%
EDTA Plasma(n=5)	91-103%	86-92%	88-97%	89-97%
Heparin Plasma(n=5)	79-94%	87-96%	78-93%	82-90%

Standard Curve

Concentration (pg/mL)	OD	Corrected OD
5000.00	2.027	1.931
2500.00	1.673	1.577
1250.00	1.243	1.147
625.00	0.935	0.839
312.50	0.573	0.477
156.25	0.315	0.219
78.13	0.258	0.162
0.00	0.096	0.000

Sample Type

Tissue homogenates, Other biological fluids

Limit Of Detection

5000 pg/mL

Precision

Matrices listed below were spiked with certain level of recombinant KIF5B and the recovery rates were calculated by comparing the measured value to the expected amount of KIF5B in samples.

Matrix	Recovery range	Average
serum(n=5)	79-97%	88%
EDTA Plasma(n=5)	80-95%	87%
Heparin Plasma(n=5)	84-98%	91%

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KIF5B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KIF5B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KIF5B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KIF5B in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Manual
MSDS

Research Area	Signal transduction;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15209215	ELK0377	48T	$320		Add to Cart
BHE15209215	ELK0377	96T	$458		Add to Cart

Human KIF5B(Kinesin Family Member 5B) ELISA Kit

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