Human M-phase Inducer Phosphatase 2

Mfr.No.

E5692Hu

Sensitivity

9.76ng/L

Detection Range

20-3500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

CDC 25B;CDC25B;Dual specificity phosphatase Cdc25B;M-phase inducer phosphatase 2

Molecular Weight

64987

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E5692Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	550.6	21.99	4
Intra-Assay	2	18	920.4	41.44	4.5
Intra-Assay	3	18	1933.2	128.38	6.6

Background

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.

Protocol

These standard curves of E5692Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2000ng/L	1.692	1.706	1.654
	1.719
1000ng/L	1.075	1.094	1.042
	1.113
500ng/L	0.501	0.497	0.445
	0.492
250ng/L	0.232	0.232	0.18
	0.231
125ng/L	0.118	0.099	0.047
	0.08
0ng/L	0.049	0.052	0
	0.054

Gene ID	994
UniProt ID	P30305

Research Area	Cell Biology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107054	E5692Hu	48T	$320		Add to Cart
BHE12107054	E5692Hu	96T	$458		Add to Cart

Human M-phase Inducer Phosphatase 2 - CDC25B ELISA Kit

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