Human Melanoma-associated Antigen D4

Mfr.No.

E5658Hu

Sensitivity

9.87ng/L

Detection Range

20-4500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

MAGE1;MAGED4;MAGE-D4;MAGE-D4 antigen;MAGEE1;MAGE-E1;MAGE-E1 antigen;Melanoma-associated antigen D4

Molecular Weight

81378

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E5658Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1966.14	34.18	1.74
Intra-Assay	2	18	552.48	31.95	5.78
Intra-Assay	3	18	1323.13	44.85	3.39

Background

May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex.

Protocol

These standard curves of E5658Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	1.978	2.011	1.953
	2.045
1200ng/L	0.987	0.959	0.9
	0.93
600ng/L	0.567	0.534	0.475
	0.5
300ng/L	0.223	0.203	0.144
	0.183
150ng/L	0.095	0.121	0.062
	0.146
0ng/L	0.06	0.058	0
	0.057

Gene ID	728239, 81557
UniProt ID	Q96JG8

Research Area	Tags & Cell Markers

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107020	E5658Hu	48T	$320		Add to Cart
BHE12107020	E5658Hu	96T	$458		Add to Cart

Human Melanoma-associated Antigen D4 - MAGED4 ELISA Kit

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