Human MTRNR2L6(Humanin-Like Protein 6) ELISA Kit

Mfr.No.

ELK8767

Sensitivity

0.151 ng/mL

Detection Range

0.32-20 ng/mL

Reactivity

Human

Assay Duration

Assay Type

Competitive Inhibition

Alternative Names

MTRNR2L6; Humanin-like 6; HN6; MT-RNR2-like protein 6

Recovery

Matrices listed below were spiked with certain level of recombinant MTRNR2L6 and the recovery rates were calculated by comparing the measured value to the expected amount of MTRNR2L6 in samples.

Matrix	Recovery range	Average
serum(n=5)	83-95%	89%
EDTA plasma(n=5)	79-93%	86%
Heparin plasma(n=5)	92-105%	89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MTRNR2L6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	98-107%	93-102%	97-103%	91-99%
EDTA plasma(n=5)	93-102%	90-99%	95-106%	87-101%
Heparin plasma(n=5)	81-97%	85-96%	78-90%	91-99%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
20.00	0.191
10.00	0.367
5.00	0.611
2.50	0.812
1.25	1.258
0.63	1.501
0.32	1.624
0.00	2.232

Sample Type

Cell lysates, Serum, Cell culture supernatants, Tissue homogenates, Plasma, Other biological fluids

Limit Of Detection

20 ng/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human MTRNR2L6 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MTRNR2L6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MTRNR2L6 in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

UniProt ID	P0CJ73

Applications	ELISA
Research Area	neuroprotective ,antiapoptotic

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SKU	Supplier No.	Size	Your price	Quantity
BHE15200180	ELK8767	48T	$320		Add to Cart
BHE15200180	ELK8767	96T	$458		Add to Cart

Human MTRNR2L6(Humanin-Like Protein 6) ELISA Kit

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