Human NEFH(Neurofilament Heavy Polypeptide) ELISA Kit

Mfr.No.

ELK0657

Sensitivity

6.81 pg/mL

Detection Range

15.63-1000 pg/mL

Reactivity

Human

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

CMT2CC; NFH

Recovery

Matrices listed below were spiked with certain level of recombinant NEFH and the recovery rates were calculated by comparing the measured value to the expected amount of NEFH in samples.

Matrix	Recovery range	Average
serum(n=5)	92-105%	98%
EDTA plasma(n=5)	84-96%	90%
Heparin plasma(n=5)	80-95%	87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NEFH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	85-94%	90-99%	82-101%	91-102%
EDTA plasma(n=5)	85-92%	89-103%	97-105%	91-99%
Heparin plasma(n=5)	88-97%	86-92%	95-103%	98-106%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
1000.00	1.978	1.880
500.00	1.534	1.436
250.00	1.165	1.067
125.00	0.895	0.797
62.50	0.528	0.430
31.25	0.385	0.287
15.63	0.156	0.058
0.00	0.098	0.000

Sample Type

Serum, Tissue homogenates, Plasma, Other biological fluids

Limit Of Detection

1000 pg/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NEFH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NEFH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NEFH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NEFH in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

UniProt ID	P12036

Applications	ELISA
Research Area	Neuro science;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15208613	ELK0657	48T	$320		Add to Cart
BHE15208613	ELK0657	96T	$458		Add to Cart

Human NEFH(Neurofilament Heavy Polypeptide) ELISA Kit

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